mmlv reverse transcriptase protocol

mmlv reverse transcriptase protocol


pH 7.6 @ 25°C. Check your inbox to complete email verification. Complete Protocol The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]. Download a PDF containing pricing for our full product list. This includes personalizing content and advertising.

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0000039826 00000 n ), DNA Modifying Enzymes & Cloning Technologies, DNA Assembly, Cloning and Mutagenesis Kits, Protein Expression & Purification Technologies, SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, Thermostable Ligase Reaction Temperature Calculator. 0 We've detected that you are using an older version of Internet Explorer.

Our customer and technical support experts are here to help! PDF (110k).

Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. The optimized coding sequence for mature MMLV-RT was cloned into pET28a and over-expressed as an N-terminal His6-tagged fusion protein. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). After that, you will need to contact Customer Service to unlock your account. 0000000016 00000 n Is M-MuLV Reverse Transcriptase active at temperatures higher than 42°C?

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Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8. M-MuLV Reverse Transcriptase synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. There was an issue logging into your account. Please try again or contact Customer Service. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. x�bbd```b``� This plasmid is available through Addgene. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version], The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. Incubate at 42°C, 1X M-MuLV Reverse Transcriptase Reaction Buffer 0000083585 00000 n You have not verified your email address. Plasmid pET-19b_MMLV-RT from Dr. Drew Endy's lab contains the insert MMLV Reverse Transcriptase. A verification email has been sent to the primary email address associated with your account. 0000005952 00000 n (1-4). 50% Glycerol Your commerce experience may be limited. Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). 0000004531 00000 n When you select your country, you agree that we can place these functional cookies on your device. 0000100224 00000 n Complete Protocol There was an issue with the password reset process. 50 mM Tris-HCl Instructions for Use of Product (s) M1701, M1705. Contact our Customer Service Team by Further information regarding NEB product quality can be found, The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. Application protocol - first-strand cDNA synthesis [ 20 uL reaction volume ] Step 1 Total RNA or RNA Æ 1 ug total RNA or 5 ng-100 ng RNA Oligo dT (or random primer) 10 – 100 pmoles DEPC water( RNase - and DNase – … M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M-MLV RT is weaker than the commonly used AMV reverse transcriptase. Please try again or contact Customer Service. 173 0 obj <> endobj endstream endobj startxref Please try again or contact Customer Service. M-MLV RT is the preferred reverse transcriptase for long mRNA templates because the RNase H activity of M-MLV RT is weaker than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase. Get in touch with a nearby distributor or sales representative, Instructions for Use of Product(s) PDF (110k). �nLD#��^���y71������v�Z�]�˻wQ�=\5&�;^�e%���c�cr� ,�������dzV=��~Q�P�Ǘ��W����Z����������Z�����/�+>v��z~��WV�`���o�m�����/����]4�=V?��f�����F̎����~zؾ|rA���]x�jOi�x>[���������n1���=������ӓ��-���'�wˑ���������xd����a;[�nC_R������)�E�&�Y�:�ս���ި������qw)#`m6�����u���L��ޝ������[�����d�]�R�C�L=W/�w��A��&j�Roԡ:���g�Uo��)��#7?_�w�Xj���jy2Y����U+LעE��\���_� ���Λ��˲�ڞM�+v��JF�l������ømʒ�r�J�N�A��e�b�[��qF���)� ���>��U� \F��|�g��������r@���pˆ50ǵ�����Xݟ,[jj��M��`g?��M�G-@#�.�VRտÐ���LV���n����r~:��-&Y�4���!�hDS�0�8�����K�w�,BcX��᠇�]6��|�}�6��dE�`/�,�KZݡ�׍kR���$]F��������X�Υ�� � -�9j?����_�p�%aNhMX�0��j.7Aw��k�>a�i�xEȀ�buy�|�Nʃ�;��+� �oY�����:�'Ҏ�c����}.�w�ul�G�K����7�)�-p}�0Rx�t(m��4P�m����uSR��g5�ؠR�8DӠ�q1xާ�Xl���y0{;�YZ}KО���:8B�t��K��w��j��ֽ������fW�m} 5��ɣ O�X�x_;��\Q�����{v�鞫�[��h�^���6��^CV]��-�ܘӀ�Z;�{걸�o�4^�a�[�SHӛ�bo_q��P����^�"�vҟp���k���w�/j;p��"�? There was an issue sending the verification email. What are some of the reasons for M-MuLV Reverse Transcriptase reaction failure? endstream endobj 174 0 obj <>/Outlines 238 0 R/Metadata 20 0 R/Pages 170 0 R/PageLayout/SinglePage/OpenAction 175 0 R/Type/Catalog>> endobj 175 0 obj <> endobj 176 0 obj <>/MediaBox[0 0 306 396]/Resources<>/ColorSpace<>/Font<>/ProcSet[/PDF/Text/ImageC/ImageI]>>/Type/Page>> endobj 177 0 obj <>stream ֗�4��5Y_�oQ?2B��E���@��"�փ��N�t������q~r4p ]CaEWW9�h>� ��A@7� B��AX��� �nz�`��;I tDB�k��. 0 0000039583 00000 n

0000010466 00000 n Note: You will not be able to access your account until your email is verified. 166 40 Roth, M.J., Tanese, N. and Goff, S.P.

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